原装ELISA试剂盒供应商 小鼠血栓素B2ELISA试剂盒公司 上海ELISA试剂盒代测
小鼠血栓素B2(TXB2)酶联免疫分析（ELISA）试剂盒
实验目的：本试剂盒仅供研究使用，用于测定小鼠血清，血浆及相关液体样本中血栓素B2(TXB2)的含量。
实验原理：本试剂盒应用双抗体夹心法测定标本中小鼠血栓素B2(TXB2)水平。用纯化的小鼠血栓素B2(TXB2)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入血栓素B2(TXB2)，再与HRP标记的血栓素B2(TXB2)抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的血栓素B2(TXB2)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中小鼠血栓素B2(TXB2)浓度。
Drug Names
Generic Name：Mouse Thromboxane B2 (TXB2) ELISA Kit.
Purpose
This kit allows for the determination of TXB2 concentrations in Mouse serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Mouse TXB2 level in the sample，use Purified Mouse TXB2 antibody to coat microtiter plate wells, make solid-phase antibody, then add TXB2 to wells, Combined TXB2 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TXB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
备注：仅供科研实验使用，具体产品信息请来电或在线方式咨询。
上海华壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd



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